Journal: International Journal of Genomics

Article Title: Single‐Cell Multiomics Integration Reveals Cell Trajectories and Regulatory Networks in Breast Cancer Progression

doi: 10.1155/ijog/9324717

Figure Lengend Snippet: Quantitative RT‐PCR validation of key subtype marker genes in breast cancer cell lines. Bar chart of relative mRNA expression levels of six marker genes (ERBB2, ESR1, PGR, KRT5, VIM, and CDH1) normalized to GAPDH in MCF‐7 (luminal, blue bars) and MDA‐MB‐231 (basal/TNBC, red bars) cell lines. Error bars represent standard deviation from three independent biological replicates. Significance brackets indicate: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by two‐tailed Student′s t ‐test. Luminal markers ERBB2, ESR1, PGR, and CDH1 show significantly higher expression in MCF‐7, whereas basal/mesenchymal markers KRT5 and VIM are significantly enriched in MDA‐MB‐231, validating single‐cell sequencing findings.

Article Snippet: MCF‐7 (luminal, ER+/PR+/HER2‐, ATCC HTB‐22) and MDA‐MB‐231 (basal/TNBC, ATCC HTB‐26) cell lines were cultured in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 μ g/mL streptomycin at 37°C/5% CO2.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Marker, Expressing, Standard Deviation, Two Tailed Test, Single-Cell Sequencing

Journal: Plants

Article Title: Chemical Characterization and In Vitro Biological Activities of Essential Oil from Oxalis corniculata L. Collected in the Ecuador Highlands

doi: 10.3390/plants15193047

Figure Lengend Snippet: Cytotoxicity of OcEO against tumors and non-tumor human cell lines. Dose–response curves for ( a ) MCF-7 (human breast adenocarcinoma), ( b ) HCT-116 (human colorectal carcinoma), and ( c ) MRC-5 (human normal lung fibroblasts). ( d ) IC 50 (concentration required to inhibit 50% of cell proliferation) comparison among the studied cell lines. R 2 : Goodness of fit for the sigmoidal dose–response analysis. Results are presented as mean ± SD from three independent experiments performed in triplicate. *: indicates significant differences between MRC5 and MCF-7, and between MRC5 and HCT-116 (ANOVA, followed by Dunnett’s post hoc test, p < 0.0001).

Article Snippet: The human breast adenocarcinoma cell line MCF-7 (ATCC, Manassas, VA, USA), the human colorectal cancer cell line HCT-116 (ECACC, Saint Louis, MO, USA), and the human normal lung fibroblast cell line MRC-5 (ABAC, Cordoba, Argentina) were cultured in DMEM (Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Internegocios, Cordoba, Argentina),100 IU/mL of penicillin and 100 μg/mL of streptomycin (GIBCO, NY, USA).

Techniques: Concentration Assay, Comparison, R-Squared, Analysis

Journal: Plants

Article Title: Chemical Characterization and In Vitro Biological Activities of Essential Oil from Oxalis corniculata L. Collected in the Ecuador Highlands

doi: 10.3390/plants15193047

Figure Lengend Snippet: Cytotoxic activity of OcEO.

Article Snippet: The human breast adenocarcinoma cell line MCF-7 (ATCC, Manassas, VA, USA), the human colorectal cancer cell line HCT-116 (ECACC, Saint Louis, MO, USA), and the human normal lung fibroblast cell line MRC-5 (ABAC, Cordoba, Argentina) were cultured in DMEM (Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (Internegocios, Cordoba, Argentina),100 IU/mL of penicillin and 100 μg/mL of streptomycin (GIBCO, NY, USA).

Techniques: Activity Assay

Journal: Molecules

Article Title: Phytochemical Analysis and Biological Activities of Essential Oils from Leaves and Stems of Dicranopteris pedata (Houtt.) Nakaike

doi: 10.3390/molecules31193518

Figure Lengend Snippet: Cytotoxicity (IC 50 μg/mL) of LEO and SEO.

Article Snippet: A panel of human cancer cell lines—HepG2 ATCC HB-8065 (liver), MCF-7 ATCC HTB-22 (breast), A-549 ATCC CCL-185 (lung), and HCT-116 ATCC CCL-247 (colon)—along with the normal hepatic line LO2 (RRID CVCL_6926) was acquired from the Shanghai Institute for Biological Sciences (SIBS), located in Shanghai, China.

Techniques:

Journal: Molecules

Article Title: Phytochemical Analysis and Biological Activities of Essential Oils from Leaves and Stems of Dicranopteris pedata (Houtt.) Nakaike

doi: 10.3390/molecules31193518

Figure Lengend Snippet: Selectivity index (SI) of LEO and SEO against different cell lines.

Article Snippet: A panel of human cancer cell lines—HepG2 ATCC HB-8065 (liver), MCF-7 ATCC HTB-22 (breast), A-549 ATCC CCL-185 (lung), and HCT-116 ATCC CCL-247 (colon)—along with the normal hepatic line LO2 (RRID CVCL_6926) was acquired from the Shanghai Institute for Biological Sciences (SIBS), located in Shanghai, China.

Techniques: